Thermo Scientific Hypersil BDS 色譜柱自 1989 年發(fā)明以來，一直廣受業(yè)界推崇，被認(rèn)為是最耐用、重現(xiàn)性**、最可信賴的色譜柱之一。Hypersil BDS 色譜柱以高度堿性去活硅膠為擔(dān)體，采用封端技術(shù)屏蔽硅醇基， 具有以下優(yōu)點：
* 出色的重現(xiàn)性
* 明顯改善峰拖尾
* 十分耐用，色譜柱使用壽命較長
* 不論是堿性化合物還是酸性化合物，均呈現(xiàn)wan美峰形。

已廣泛應(yīng)用在許多藥典方法中，例如：一枝黃花（蘆丁)、連翹（連翹酯苷A）、益母草（鹽酸益母草堿）、三黃片（大黃素）、小兒消食片（熊果酸）、牛黃清宮丸（黃芩苷）、風(fēng)濕骨痛膠囊（鹽酸）、芎菊上清片（黃芩苷）、益母生膠囊（芍藥苷）、葛根芩連片（葛根素）等等。

為什么要用堿性去活硅膠和鍵合相？

使用共價鍵合硅膠固定相，可對多數(shù)化合物進(jìn)行分離。HPLC 與液-液分配色譜相比，具有平衡速度快、分配性能更好的特點，因此，HPLC 作為現(xiàn)代分析手段被廣泛應(yīng)用。

但是，傳統(tǒng)的共價鍵合硅膠固定相存在以下問題：
HPLC 中使用的建和硅膠固定相與分析物之間存在多種相互作用，這些相互作用一部分是鍵合相本身與分析物之間的作用，另一部分是鍵合時在硅膠表面殘留的硅醇基與分析物之間的作用。

硅醇基的數(shù)目和酸性極大程度影響了它與分析物間相互作用的強(qiáng)弱。堿性硅膠的酸-堿相互作用主要是由硅醇基產(chǎn)生的，硅醇基還決定了硅膠填料的表面極性。硅醇基的種類和酸性在很大程度上影響了 HPLC 的分離度和分析物的色譜峰形。
殘余硅醇基與化合物的相互作用會使酸性和堿性化合物的色譜峰產(chǎn)生拖尾。早期使用的 HPLC 硅膠填料，硅醇基具有很強(qiáng)的酸性，因此色譜拖尾現(xiàn)象特別明顯。

使用早期硅膠填料時，若要得到較好的色譜峰，通常需要在流動相中添加酸（醋酸）或堿（三乙胺），使其與硅醇基產(chǎn)生競爭性結(jié)合，從而改善色譜峰形。當(dāng)使用了合適的流動相添加劑時，色譜峰形會得到明顯改善。通常需要很高的添加劑濃度多數(shù)情況下達(dá)到 1% （體積比）或更高，這樣才能使所有殘留硅醇基都與添加劑結(jié)合，而不影響分析物的色譜峰形。但是高濃度添加劑常常嚴(yán)重降低色譜柱壽命和方法重現(xiàn)性。

所以Hypersil BDS 是的堿性去活硅膠之一，具有以下優(yōu)點：
* 減弱硅醇基相互作用
* 改善色譜峰拖尾
* 不需向流動相中加入添加劑
* 的色譜峰對稱性
* 延長色譜柱使用壽命
* 提高柱效（分析堿性、酸性、中性化合物）

Hypersil BDS重現(xiàn)性，批次間測試流程，多種固定相：

Hypersil BDS 色譜柱是根據(jù)最高標(biāo)準(zhǔn)生產(chǎn)的，并進(jìn)過嚴(yán)苛的質(zhì)量控制。硅膠擔(dān)體和色譜柱的生產(chǎn)流程均完全遵守 ISO9001:2000 標(biāo)準(zhǔn)。在進(jìn)行鍵合前，BDS 硅膠必須通過 30 余項物理特性和色譜性能測試。在鍵合后，所有的 BDS C18 填料都經(jīng)過色譜性能測試以及碳載量測試該測試在封端前和封端后各進(jìn)行一次。

Hypersil BDS 色譜柱有 4 種鍵合相供選擇。所有 Hypersil BDS 色譜柱，均使用堿性去活工藝處理硅膠擔(dān)體，再經(jīng)封端處理屏蔽硅醇基，即使是分析堿性化合物，也極大程度改善了峰拖尾。每支 Hypersil BDS 色譜柱都帶有《質(zhì)量認(rèn)證書》

Hypersil BDS填料參數(shù)

填料 顆粒形狀/大小（um） 孔徑(?) 表面積(m2/g) 碳載量% 端基封尾 USP PH 范圍
Hypersil BDS C18 Spher. 2.4，3, 5 130 170 11 Yes L1 2-9
Hypersil BDS C8 Spher. 2.4，3, 5 130 170 7 Yes L7 2-9
Hypersil BDS Phenyl Spher. 2.4，3, 5 130 170 5 Yes L11 2-9
Hypersil BDS CN Spher. 2.4，3, 5 130 170 4 Yes L10 2-9


Hypersil BDS供貨信息

貨號 描述
28102-012101 10x2.1mm 2.4um Hypersil BDS C18

28102-052130 50x2.1mm 2.4 um Hypersil BDS C18

28102-054630 50x4.6mm 2.4 umHypersil BDS C18
28102-102130 100x2.1mm 2.4 um Hypersil BDS C18
28102-104630 100x4.6mm 2.4 um Hypersil BDS C18
28102-152130 150x2.1mm 2.4 umHypersil BDS C18
28102-154630 150x4.6mm 2.4 umHypersil BDS C18
28103-012101 10x2.1mm 3um BDS C18 保護(hù)柱柱芯
28103-013001 10x3mm 3um BDS C18 保護(hù)柱柱芯
28103-014001 10x4mm 3um BDS C18 保護(hù)柱柱芯
28103-052130 50x2.1mm 3umHypersil BDS C18
28103-053030 50x3mm 3umHypersil BDS C18
28103-054030 50x4mm 3umHypersil BDS C18
28103-054630 50x4.6mm 3um BDS C18
28103-102130 100x2.1mm 3um BDS C18
28103-104030 100x4mm 3um BDS C18
28103-104630 100x4.6mm 3um BDS C18
28103-124030 125x4mm 3u BDS C18
28103-124630 125x4.6mm 3u BDS C18
28103-152130 150x2.1mm 3um BDS C18
28103-154630 150x4.6mm 3um BDS C18
28103-204630 200x4.6mm 3u BDS C18
28103-252130 250x2.1mm 3u Hypersil BDS C18
28103-253030 250x3mm 3u Hypersil BDS C18
28103-254030 250x4mm 3u Hypersil BDS C18
28103-254630 250x4.6mm 3u Hypersil BDS C18
28105-011001 10x1mm 5um Hypersil BDS C18 保護(hù)柱柱芯
28105-012101 10x2.1mm 5um Hypersil BDS C18 保護(hù)柱柱芯
28105-012106 10x2.1mm 5u BDS C18 Javelin 直連式保護(hù)柱
28105-013001 10x3mm 5um BDS C18 保護(hù)柱柱芯
28105-014001 10x4mm 5um BDS C18 保護(hù)柱柱芯
28105-052130 50x2.1mm 5um Hypersil BDS C18
28105-054630 50x4.6mm 5um Hypersil BDS C18
28105-102130 100x2.1mm 5umHypersil BDS C18
28105-104630 100x4.6mm 5um Hypersil BDS C18
28105-122130 125x2.1mm 5umHypersil BDS C18
28105-124030 125x4mm 5umHypersil BDS C18
28105-124630 125x4.6mm 5umHypersil BDS C18
28105-152130 150x2.1mm 5umHypersil BDS C18
28105-153030 150x3mm 5um Hypersil BDS C18
28105-154030 150x4mm 5umHypersil BDS C18
28105-154630 150x4.6mm 5umHypersil BDS C18
28105-159270 150x21.2mm 5u Hypersil BDS C18 制備柱
28105-202130 200x2.1mm 5umHypersil BDS C18
28105-204030 200x4mm 5umHypersil BDS C18
28105-204630 200x4.6mm 5um Hypersil BDS C18
28105-252130 250x2.1mm 5um Hypersil BDS C18
28105-252168 250x2.1mm 5umHypersil BDS C18 PEEK柱
28105-254030 250x4mm 5um Hypersil BDS C18
28105-254630 250x4.6mm 5um Hypersil BDS C18
28105-259070 250x10mm 5um BDS C18 制備柱
28105-259270 250x21.2mm 5um BDS C18 制備柱
28105-304030 300x4mm 5um BDS C18
28105-304630 300x4.6mm 5um BDS C18
28202-012101 10x2.1mm 2.4um BDS C8
28202-014001 10x4mm 2.4um Hypersil BDS C8
28202-052130 50x2.1mm 2.4 um Hypersil BDS C8
28202-054630 50x4.6mm 2.4 um Hypersil BDS C8
28202-102130 100x2.1mm 2.4 um Hypersil BDS C8
28202-104630 100x4.6mm 2.4 um Hypersil BDS C8
28202-152130 150x2.1mm 2.4 um Hypersil BDS C8
28202-154630 150x4.6mm 2.4 um Hypersil BDS C8
28203-012101 10x2.1mm, 3um Hypersil Hypersil BDS C8 保護(hù)柱柱芯
28203-013001 10x3mm, 3um Hypersil Hypersil BDS C8 保護(hù)柱柱芯
28203-014001 10x4mm, 3um Hypersil Hypersil BDS C8 保護(hù)柱柱芯
28203-032130 30x2.1mm 3u Hypersil BDS C8
28203-033030 30x3mm 3u Hypersil BDS C8
28203-034630 30x4.6mm 3u Hypersil BDS C8
28203-052130 50x2.1mm 3um Hypersil BDS C8
28203-053030 50x3mm 3um Hypersil BDS C8
28203-054030 50x4mm 3um Hypersil BDS C8
28203-054630 50x4.6mm 3um Hypersil BDS C8
28203-074630 75x4.6mm 3u Hypersil BDS C8 column
28203-102130 100x2.1mm 3um Hypersil Hypersil BDS C8
28203-103030 100x3mm 3um Hypersil Hypersil BDS C8
28203-104030 100x4mm 3um Hypersil Hypersil BDS C8
28203-104630 100x4.6mm 3um Hypersil Hypersil BDS C8
28203-123030 125x3mm 3u Hypersil BDS C8
28203-124630 125x4.6mm 3u Hypersil BDS C8
28203-152130 150x2.1mm 3um Hypersil Hypersil BDS C8
28203-153030 150x3mm 3um Hypersil Hypersil BDS C8
28203-154030 150x4mm 3um Hypersil Hypersil BDS C8
28203-154630 150x4.6mm 3um Hypersil Hypersil BDS C8
28203-252130 250x2.1mm 3u Hypersil BDS C8
28203-254630 250x4.6mm 3u Hypersil BDS C8
28205-011001 10x1mm, 5um Hypersil Hypersil BDS C8 保護(hù)柱柱芯
28205-012101 10x2.1mm, 5um Hypersil Hypersil BDS C8 保護(hù)柱柱芯
28205-032130 30x2.1mm 5u Hypersil BDS C8
28205-034630 30x4.6mm 5u Hypersil BDS C8
28205-052130 50x2.1mm 5um Hypersil BDS C8
28205-053030 50x3mm 5um Hypersil BDS C8
28205-054030 50x4mm 5um Hypersil Hypersil BDS C8
28205-054630 50x4.6mm 5um Hypersil Hypersil BDS C8
28205-102130 100x2.1mm 5um Hypersil Hypersil BDS C8
28205-103030 100x3mm 5um Hypersil Hypersil BDS C8
28205-104030 100x4mm 5um Hypersil Hypersil BDS C8
28205-104630 100x4.6mm 5um Hypersil Hypersil BDS C8
28205-124030 125x4mm 5u Hypersil BDS C8
28205-124630 125x4.6mm 5u Hypersil BDS C8
28205-152130 150x2.1mm 5um Hypersil Hypersil BDS C8
28205-153030 150x3mm 5um Hypersil Hypersil BDS C8
28205-154030 150x4mm 5um Hypersil Hypersil BDS C8
28205-154630 150x4.6mm 5um Hypersil Hypersil BDS C8
28205-159070 150x10mm 5u Hypersil BDS C8 制備柱
28205-204630 200x4.6mm 5u Hypersil BDS C8
28205-252130 250x2.1mm 5um Hypersil Hypersil BDS C8
28205-253030 250x3mm 5um Hypersil Hypersil BDS C8
28205-254030 250x4mm 5um Hypersil Hypersil BDS C8
28205-254630 250x4.6mm 5um Hypersil Hypersil BDS C8
28205-304030 300x4mm 5u Hypersil Hypersil BDS C8
28802-052130 50x2.1mm 2.4 um Hypersil BDS CN
28802-054630 50x4.6mm 2.4 um Hypersil BDS CN
28802-102130 100x2.1mm 2.4 um Hypersil BDS CN
28802-152130 150x2.1mm 2.4 um Hypersil BDS CN
28803-012101 10x2.1mm, 3um Hypersil Hypersil BDS Cyano 保護(hù)柱柱芯
28803-052130 50x2.1mm 3um Hypersil Hypersil BDS Cyano
28803-054630 50x4.6mm 3um Hypersil Hypersil BDS Cyano
28803-102130 100x2.1mm 3um Hypersil Hypersil BDS Cyano
28803-104630 100x4.6mm 3um Hypersil Hypersil BDS Cyano
28803-152130 150x2.1mm 3um Hypersil Hypersil BDS Cyano
28803-153030 150x3mm 3um Hypersil Hypersil BDS Cyano
28803-154030 150x4mm 3um Hypersil Hypersil BDS Cyano
28803-154630 150x4.6mm 3um Hypersil Hypersil BDS Cyano
28805-012101 10x2.1mm, 5um Hypersil Hypersil BDS Cyano 保護(hù)柱柱芯
28805-014001 10x4mm, 5um Hypersil Hypersil BDS Cyano 保護(hù)柱柱芯

28805-052130 50x2.1mm 5um Hypersil Hypersil BDS Cyano
28805-053030 50x3mm 5um Hypersil Hypersil BDS Cyano
28805-054030 50x4mm 5um Hypersil Hypersil BDS Cyano
28805-054630 50x4.6mm 5um Hypersil Hypersil BDS Cyano
28805-102130 100x2.1mm 5um Hypersil Hypersil BDS Cyano
28805-103030 100x3mm 5um Hypersil Hypersil BDS Cyano
28805-104030 100x4mm 5um Hypersil Hypersil BDS Cyano
28805-104630 100x4.6mm 5um Hypersil Hypersil BDS Cyano
28805-152130 150x2.1mm 5um Hypersil Hypersil BDS Cyano
28805-153030 150x3mm 5um Hypersil Hypersil BDS Cyano
28805-154030 150x4mm 5um Hypersil Hypersil BDS Cyano
28805-154630 150x4.6mm 5um Hypersil Hypersil BDS Cyano
28805-204630 200x4.6mm 5u Hypersil BDS CPS
28805-252130 250x2.1mm 5um Hypersil BDS Cyano
28805-254030 250x4mm 5um Hypersil Hypersil BDS Cyano
28805-254630 250x4.6mm 5um Hypersil Hypersil BDS Cyano
28805-304030 300x4mm 5u Hypersil Hypersil BDS CPS
28805-304630 300x4.6mm 5u Hypersil BDS CPS
28902-052130 50x2.1mm 2.4um Hypersil BDS Phenyl
28902-054630 50x4.6mm 2.4um Hypersil BDS Phenyl
28902-102130 100x2.1mm 2.4u Hypersil BDS Phenyl
28902-154630 150x4.6mm 2.4u Hypersil BDS Phenyl
28903-012101 10x2.1mm, 3um Hypersil Hypersil BDS Phenyl 保護(hù)柱柱芯
28903-052130 50x2.1mm 3um Hypersil Hypersil BDS Phenyl
28903-054630 50x4.6mm 3um Hypersil Hypersil BDS Phenyl
28903-102130 100x2.1mm 3um Hypersil Hypersil BDS Phenyl
28903-104030 100x4mm 3um Hypersil Hypersil BDS Phenyl
28903-104630 100x4.6mm 3um Hypersil Hypersil BDS Phenyl
28903-152130 150x2.1mm 3um Hypersil Hypersil BDS Phenyl
28903-153030 150x3mm 3um Hypersil Hypersil BDS Phenyl
28903-154030 150x4mm 3um Hypersil Hypersil BDS Phenyl
28903-154630 150x4.6mm 3um Hypersil Hypersil BDS Phenyl
28905-012101 10x2.1mm, 5um Hypersil Hypersil BDS Phenyl 保護(hù)柱柱芯
28905-014001 10x4mm, 5um Hypersil Hypersil BDS Phenyl 保護(hù)柱柱芯
28905-052130 50x2.1mm 5um Hypersil Hypersil BDS Phenyl
28905-054630 50x4.6mm 5um Hypersil Hypersil BDS Phenyl
28905-102130 100x2.1mm 5um Hypersil Hypersil BDS Phenyl
28905-104030 100x4mm 5um Hypersil Hypersil BDS Phenyl
28905-104630 100x4.6mm 5um Hypersil Hypersil BDS Phenyl
28905-152130 150x2.1mm 5um Hypersil Hypersil BDS Phenyl
28905-154030 150x4mm 5um Hypersil Hypersil BDS Phenyl
28905-154630 150x4.6mm 5um Hypersil Hypersil BDS Phenyl
28905-204630 200x4.6mm 5u Hypersil BDS Phenyl
28905-252130 250x2.1mm 5um BDS Phenyl
28905-254030 250x4mm 5um BDS Phenyl
28905-254630 250x4.6mm 5um BDS Phenyl