描述 Thermo Scientific Pierce Black, Protein G Coated 96-Well Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

Features of Protein G Coated Plates:

? Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
? Orient antibodies for maximum antigen-binding capacity
? Immobilize antibodies for plate assays without prior purification
? Ensure minimal variation (<5% well-to-well) from consistent coating
? Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
? Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

These plates are uniformly and stably coated with Protein G, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Protein G Coated Microplates:
? Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat
? Binds only to IgG—no cross-reactivity with other antibody classes
? Binds to Fc region of antibodies for optimal orientation

Related Products
Pierce? Protein G Coated Plates, Clear, 96-Well
Pierce? Protein G Coated Plates, Clear, 8-Well Strip
Pierce? Protein G Coated Plates, White, 96-Well

For Research Use Only. Not for use in diagnostic procedures.

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