Synonyms

IL-1RN, IRAP

Accession

P18510

GeneID

3557

Source

E.coli-derived Human IL-1RA, Arg26-Glu177.

Molecular Weight

Approximately 26.2 kDa.

AA Sequence

RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 96% by SDS-PAGE and HPLC analyses.

Biological Activity

The ED₅₀ as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 40 ng/mL, corresponding to a specific activity of > 2.5 × 10⁴ IU/mg in the presence of 50 pg/ml rHuIL-1α. Fully biologically active when compared to standard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.